| Women's Singles | |
|---|---|
| 1965 Australian Championships | |
| Champion |
|
| Runner-up |
|
| Final score | 5-7, 6-4, 5-2 ret. |
| Draw | 52 |
| Seeds | 16 |
Margaret Smith defeated Maria Bueno 5-7, 6-4, 5-2 ret. in the final to win the Women's Singles title at the 1965 Australian Championships.
The seeded players are listed below. Margaret Smith is the champion; others show the round in which they were eliminated.