| FA Challenge Trophy | |||
| Country |
|
||
|---|---|---|---|
| Teams | 175 | ||
| Champions | Canvey Island | ||
| Runners-up | Forest Green Rovers | ||
|
|||
The 2000–01 FA Trophy was the thirty-second season of the FA Trophy.
The competition was won by Essex-side Canvey Island, who defeated Forest Green Rovers 1–0 in the Final at Villa Park, Birmingham.