| Singles | |
|---|---|
| 2000 WTA Madrid Open | |
| Champion |
|
| Runner-up |
|
| Final score | 4–6, 6–2, 6–2 |
Lindsay Davenport was the defending champion, but did not compete this year.
Gala León García won the title by defeating Fabiola Zuluaga 4–6, 6–2, 6–2 in the final. It was the 1st and only title in her career.
The first two seeds received a bye into the second round.