The 2010 football season was São Paulo's 81st season since club's existence. In this year Tricolor take part on its seventh consecutive Copa Libertadores however was defeated at semifinals by Internacional, that would be champion later, on away goal rule due a goal scored by opponent on Morumbi, 0–1 (away); 2–1 (home). The club came third in Campeonato Paulista losing at semifinals to rival Santos. In Série A ended in ninth position reaching a qualifying to Copa Sudamericana breaking the sequence of qualification to the Copa Libertadores, since 2004.

As of 21 July 2010Note: Flags indicate national team as defined under FIFA eligibility rules. Players may hold more than one non-FIFA nationality.

Note: Flags indicate national team as defined under FIFA eligibility rules. Players may hold more than one non-FIFA nationality.