| Singles | |
|---|---|
| 2016 J&T Banka Prague Open | |
| Champion |
|
| Runner-up |
|
| Final score | 3–6, 6–1, 6–4 |
Karolína Plíšková was the defending champion, but lost in the semifinals to Lucie Šafářová.
Šafářová went on to win the title, defeating Samantha Stosur in the final, 3–6, 6–1, 6–4.