2016 Knoxville Challenger – Singles
| Singles | |
|---|---|
| 2016 Knoxville Challenger | |
| Champion |
 Michael Mmoh
|
| Runner-up |
 Peter Polansky
|
| Final score | 7–5, 2–6, 6–1 |
Daniel Evans was the defending champion but lost in the second round to Peter Polansky.
Michael Mmoh won the title after defeating Polansky 7–5, 2–6, 6–1 in the final.
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