| Country | Germany | ||
|---|---|---|---|
| Teams | 32 | ||
| Defending champions | FFC Frankfurt | ||
| Champions | FFC Frankfurt | ||
| Runners-up | Sportfreunde Siegen | ||
| Matches played | 28 | ||
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The Frauen DFB-Pokal 1999–2000 was the 20th season of the cup competition, Germany's second-most important title in women's football. The first round of the competition was held on 22–25 August 1999. In the final which was held in Berlin on 6 May 2000 FFC Frankfurt defeated Sportfreunde Siegen 2–1, thus defending their title from the previous seasons and claiming their second title.
* Windhorst Drosendorf and Ahrbach withdrew their teams. Nuremberg and Saarbücken thus advanced to the next round.
* Hertha Zehlendorf withdrew their team. Brauweiler Pulheim thus advanced to the next round.