The New Castle District is a railroad line owned and operated by the Norfolk Southern Railway in the U.S. states of Ohio and Indiana. The line runs from Cincinnati, Ohio northwest and north to Fort Wayne, Indiana along former Pennsylvania Railroad and New York, Chicago and St. Louis Railroad (Nickel Plate) lines. Its south end is at Evendale, where it meets the Dayton District and Indiana and Ohio Railway's Oasis Subdivision. It runs along a piece of CSX Transportation's Toledo Subdivision from Hamilton north to New Miami, Ohio using trackage rights. The New Castle District junctions the Frankfort District at Muncie, Indiana. In Fort Wayne, it crosses the Huntington District and ends at the Chicago District.
The Eaton and Hamilton Railroad (in Ohio) and Richmond and Miami Railroad (in Indiana) opened a line from New Miami, Ohio northwest to Richmond, Indiana in 1853, with trackage rights over the Cincinnati, Hamilton and Dayton Railroad from New Miami south to Hamilton. Later that year, the Cincinnati, Logansport and Chicago Railway opened, extending northwest from Richmond to New Castle. From Hamilton south to Evendale and beyond to Rendcomb Junction, the Cincinnati and Richmond Railroad opened in 1888. This line from Cincinnati to New Castle became part of the Pennsylvania Railroad system through leases and mergers.